Optimization of the position of single-lead wireless sensor with low electrodes separation distance for ECG-derived respiration

A classical method for estimation of respiratory information from electrocardiogram (ECG), called ECG - derived respiration (EDR), is using flexible electrodes located at standard electrocardiography positions. This work introduces an alternative approach suitable for miniaturized sensors with low inter-electrode separation and electrodes fixed to the sensor encapsulation. Application of amplitude EDR algorithm on single-lead wireless sensor system with optimized electrode positions shows results comparable with standard robust systems. The modified method can be applied in daily physiological monitoring, in sleep studies or implemented in smart clothes when standard respiration techniques are not suitable

APPLICATION OF SINGLE WIRELESS HOLTER TO SIMULTANEOUS EMG, MMG AND EIM MEASUREMENT OF HUMAN MUSCLES ACTIVITY

This paper describes application and design of wireless holter with innovative functionality, used it in field of human muscle monitoring. In our experiments we monitored EMG (electromyography), MMG (mechanomyography) and EIM (electrical impedance myography) all by single device. It is first time when these all parameters were monitored simultaneously taking advantage of the holter device data output in order to find the signals interconnection. Our data were compared with normally used medical device and signal quality was verified

Photocycle alteration and increased enzymatic activity in genetically modified photoactivated adenylate cyclase OaPAC

Photoactivated adenylate cyclases (PACs) are light activated enzymes that combine blue light sensing capacity with the ability to convert ATP to cAMP and pyrophosphate (PPi) in a light-dependent manner. In most of the known PACs blue light regulation is provided by a blue light sensing domain using flavin which undergoes a structural reorganization after blue-light absorption. This minor structural change then is translated toward the C-terminal of the protein, inducing a larger conformational change that results in the ATP conversion to cAMP. As cAMP is a key second messenger in numerous signal transduction pathways regulating various cellular functions, PACs are of great interest in optogenetic studies. The optimal optogenetic device must be “silent” in the dark and highly responsive upon light illumination. PAC from Oscillatoria acuminata is a very good candidate as its basal activity is very small in the dark and the conversion rates increase 20-fold upon light illumination. We studied the effect of replacing D67 to N, in the blue light using flavin domain. This mutation was found to accelerate the primary electron transfer process in the photosensing domain of the protein, as has been predicted. Furthermore, it resulted in a longer lived signaling state, which was formed with a lower quantum yield. Our studies show that the overall effects of the D67N mutation lead to a slightly higher conversion of ATP to cAMP, which points in the direction that by fine tuning the kinetic properties more responsive PACs and optogenetic devices can be generated

Material Handling Equipment Selection Algorithm for Production Workplaces

Nowadays, the consumption is the most significant factor in the economy. The consumption requires different items, which must be produced by the industry. Production processes are realized mainly on workplaces, so their importance is inevitably. This paper presents an overview about the planning process of material handling machines, especially for the production workplaces. The research, shown in this paper, targets to develop a model, which can help to select the most suitable material handling equipment for different production workplaces. The topic presented in this paper deals with the building of selection algorithm for the exclusion of non-applicable equipment types. To show the useability of the concept an example will also be presented